Sample Analysis Report Date: December 20, 2005
1. Experimental Information
- Sample: Yellow-light powder, water-soluble
- Injection Volume: 50μl (5μg/μl)
- Tester: C. H. Shih
- Correspondence: Ph.D. C. H. Yen
2. Instrument Settings
- HPLC System: Waters
- Column: Superdex™ Peptide HR10/30 (Amersham Pharmacia Biotech AB)
- Wavelength: 215 nm
- Flow Rate: 0.5 ml/min
- Elution (Mobile Phase): 26.6% Acetonitrile in 0.08% TFA/H₂O
3. Standard Curve (Calibration)
The relationship between Retention Time and the logarithm of molecular weight (Log Mr) was established using standard markers:
Regression Equation: $Y = -0.1121X + 6.627$
Correlation Coefficient ($R^2$): 0.9923
Standard Markers:
- Cytochrome C: 12,500 Da
- Aprotinin: 6,500 Da
- Gastrin I: 2,126 Da
- (Gly)₆: 360 Da
- (Gly)₃: 180 Da
4. Molecular Weight Distribution Results
Theoretical distribution percentages calculated based on the Time vs. Log(MW) curve area:
| Retention Time (min) | Molecular Size (Dalton) | Distribution Percentage (%) |
| 25.0 ~ 28.0 | 6683 ~ 3076 | 1.79 |
| 28.0 ~ 30.1 | 3076 ~ 1791 | 10.10 |
| 30.1 ~ 32.0 | 1791 ~ 1096 | 22.00 |
| 32.0 ~ 34.0 | 1096 ~ 655 | 27.69 |
| 34.0 ~ 36.1 | 655 ~ 380 | 19.27 |
| 36.1 ~ 38.3 | 380 ~ 216 | 9.75 |
| 38.3 ~ 40.0 | 216 ~ 139 | 6.93 |
| 40.0 ~ 45.0 | 139 ~ 38 | 2.49 |
Summary: The molecular weight of the sample is primarily concentrated between 655 and 1791 Dalton, which accounts for 49.69% of the total distribution.
Executive Institute: Division of Biotechnology, Animal Technology Institute Taiwan (ATIT) Lab.